Laatste update: 09-03-2021

CRISPR/Cas9
A theoretical and practical approach

Course Date:  will be rescheduled soon 

This course is a collaboration between Avans Hogeschool Breda and Hogeschool Inholland Amsterdam

Aims of the course:
• Learn the basic principles of the CRISPR/Cas9 toolbox

• Learn the applications of the CRISPR/Cas9 toolbox in different model systems (human, plant and yeast/bacteria) • Learn how to design guide-RNAs

• Learn how to perform a Crispr/Cas9 experiment on human cells

• Learn how to analyze a Crispr/Cas9 experiment

• Learn about the newest tools in CRISPR/Cas technology

Participants:

This course has been developed for technicians and teachers from universities of applied sciences, and all other laboratory technicians who are interested or involved in the CRISPR/Cas9 technology. Participants are expected to bring their own laptop: (PC or Mac) as this will be needed for the course.

Course structure:  

We offer a 2-day course consisting of a series of lectures and "hands-on" experiments and computer assignments. All participants will receive a course manual.

Topics included in the course:

Day 1

Lectures:
CRISPR/Cas9, history, technology (Mutagenesis and HDR) Challenges and applications of CRISPR-Cas9 in Yeast and Bacteria
Challenges and applications of CRISPR-Cas9 in Plants

Parallel practical sessions:
CRISPR knock-out in a human cell line with GFP
computer assignment: Develop a CRISPR/Cas9 guide

Day 2

Lectures:

Challenges and applications of CRISPR-Cas9 in Human cells The Editor Edited, the latest variations in CRISPR technology Detection of on-target and off-target mutations generated by CRISPR/Cas9

Parallel practical sessions:

Analysis of the GFP knock-outs
computer assignment: Detection of mutations with TIDE and TIDER

Avans Hogeschool reserves the right to substitute speakers, and change the theoretical and practical program, in order to cover most current and future new CRISPR technology applications.

 

Course staff:

Course leaders:

Dr. M.C.M. Verschuren, Avans Hogeschool, Research group Analysis Techniques in the Life Sciences, Breda, NL

Dr. C.M. Kreike, Lector Green Biotechnology, Domein Agri, Food & Life Sciences, Hogeschool Inholland, Amsterdam, NL

Accreditation:
All participants will receive a certificate of attendance

General Information:
Code: 2547 (2-day course)

Registration fee:

Hogeschool employee: € 200,-

Other participants: € 300,-

The fee includes course materials and lunch Language: English Location:

Avans Hogeschool, Lovensdijkstraat 63, Breda, The Netherlands.